s pneumoniae d39  (ATCC)

Journal: Molecules

Article Title: Shionone-Targeted Pneumolysin to Ameliorate Acute Lung Injury Induced by Streptococcus pneumoniae In Vivo and In Vitro

doi: 10.3390/molecules27196258

Figure Lengend Snippet: Shionone inhibits PLY hemolytic activity and does not affect S. pneumoniae growth at less than 8 μg/mL. ( A ) Shionone chemical structure formula. ( B ) Shionone exhibits a concentration-dependent inhibitory effect on the hemolytic activity of PLY. ( C ) The growth curves of S. pneumoniae were determined under shionone treatment with different concentrations (0 μg/mL, 4 μg/mL and 8 μg/mL). ( D ) The fluorescence images of S. pneumoniae treated with different concentrations of shionone (0 μg/mL, 4 μg/mL, and 8 μg/mL) for 6 h. Green in the image represents live cells, and red represents dead cells. The significance level was expressed as follows: ** p < 0.01.

Article Snippet: The S. pneumoniae strain D39 (ATCC49619) used in this study was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). and cultured according to previous research [ ].

Techniques: Activity Assay, Concentration Assay, Fluorescence

Journal: Molecules

Article Title: Shionone-Targeted Pneumolysin to Ameliorate Acute Lung Injury Induced by Streptococcus pneumoniae In Vivo and In Vitro

doi: 10.3390/molecules27196258

Figure Lengend Snippet: Shionone inhibits virulence of S. pneumoniae by targeting the oligomerization of PLY. ( A ) The oligomerization of PLY was detected by western blotting treated with or without shionone. ( B ) Densitometric analysis of PLY oligomerization with different concentrations of shionone treatment. ( C ) The expression level of PLY determined after S. pneumoniae was cocultured with different concentrations of shionone for 6 h. ( D , E ) Shionone interacts with the active pocket of PLY and targets amino acid sites ASP-59, ILE-60, THR-57, PHE-344, and ASN-346. The significance level was expressed as follows: ** p < 0.01.

Article Snippet: The S. pneumoniae strain D39 (ATCC49619) used in this study was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). and cultured according to previous research [ ].

Techniques: Western Blot, Expressing

Journal: Molecules

Article Title: Shionone-Targeted Pneumolysin to Ameliorate Acute Lung Injury Induced by Streptococcus pneumoniae In Vivo and In Vitro

doi: 10.3390/molecules27196258

Figure Lengend Snippet: Shionone alleviates mice pneumonia injury induced by S. pneumoniae . ( A , B ) Gross lung tissue lesions and hematoxylin/eosin staining analysis after infected for 48 h with or without 50 mg/kg shionone treatment. ( C ) Lung colony count of the control, model, and treatment groups after infection for 48 h. The significance level was expressed as follows: ** p < 0.01; NS, no significant difference.

Article Snippet: The S. pneumoniae strain D39 (ATCC49619) used in this study was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). and cultured according to previous research [ ].

Techniques: Staining, Infection, Control

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top-Down LESA Mass Spectrometry Protein Analysis of Gram-Positive and Gram-Negative Bacteria

doi: 10.1007/s13361-017-1718-8

Figure Lengend Snippet: Liquid extraction surface analysis (LESA) mass spectra of seven bacterial strains: Escherichia coli K-12, Escherichia coli BL21 mCherry, Pseudomonas aeruginosa PS1054, Staphylococcus aureus MSSA476, Streptococcus pneumoniae D39, Streptococcus oralis ATCC 35037, and Streptococcus gordonii ATCC 35105. All mass spectra were acquired immediately following incubation of the colonies at 37 °C, with use of the 40:60:1 acetonitrile–water–formic acid solvent system for Gram-negative bacteria and the 50:45:5 variant for Gram-positive bacteria. The streptococci were incubated under semianaerobic conditions for optimum growth; the remaining strains were grown in open air

Article Snippet: As an additional challenge, colonies of three species of Streptococcus — S. pneumoniae D39, S. oralis ATCC 35037, and S. gordonii ATCC 35105—were subjected to LESA MS characterization.

Techniques: Extraction, Incubation, Solvent, Bacteria, Variant Assay

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top-Down LESA Mass Spectrometry Protein Analysis of Gram-Positive and Gram-Negative Bacteria

doi: 10.1007/s13361-017-1718-8

Figure Lengend Snippet: Proteins Identified by Liquid Extraction Surface Analysis Mass Spectrometry in Sampled Colonies

Article Snippet: As an additional challenge, colonies of three species of Streptococcus — S. pneumoniae D39, S. oralis ATCC 35037, and S. gordonii ATCC 35105—were subjected to LESA MS characterization.

Techniques: Extraction, Mass Spectrometry, Molecular Weight, Incubation

Journal:

Article Title: Cholesterol as Treatment for Pneumococcal Keratitis: Cholesterol-Specific Inhibition of Pneumolysin in the Cornea

doi: 10.1167/iovs.07-0017

Figure Lengend Snippet: Table 1

Article Snippet: Bacterial Strains and Growth Conditions S. pneumoniae D39 (Item 6302; American Type Culture Collection, Manassas, VA) was cultured on blood agar base (Oxoid; Remel, Inc., Lenexa, KS) containing 5% sheep blood (Hemostat Laboratories, Dixon, CA) at 37°C and routinely grown in Todd Hewitt broth supplemented with 0.5% yeast extract (THY; BD Biosciences, Sparks, MD) at 37°C, unless otherwise indicated.

Techniques: In Vitro